Precise genome editing in Plodia interpunctella.

Publication Cover

The eye mutation of a pantry moth Plodia interpunctella is induced by CRISPR/Cas9 targeted at white gene. CRISPR-mediated knock-out and rescue of white resulted in both bi-allelic somatic editing and germline transmission at relatively high efficiency. Our study reveals the convenience of using Plodia as a tractable lepidopteran model organism for functional genetics via precise genome editing using the CRISPR/Cas9 system. (iScience 2022 Mar 18, Vol 25, Issue 3).

Publications

Heryanto C, Ratnappan R, O'Halloran DM, Hawdon JM, Ioannis Eleftherianos. (2022) "Culturing and Genetically Manipulating Entomopathogenic Nematodes." JoVE. In production.

Heryanto C, Hanly JJ, Mazo-Vargas A, Tendolkar A, Martin A. (2022) "Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths." iScience. 25(3):103885.

Lian J, Steinert G, de Vree J, Meijer S, Heryanto C, Bosma R, Wijffels RH, Barbosa MJ, Smidt H, Sipkema D. (2022) "Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78." Appl Microbiol Biotechnol. 106(5-6):2235-2248.

Hanly JJ, Livraghi L, Heryanto C, McMillan W, Jiggins C, Gilbert L, Martin A. (2022) "A large deletion at the cortex locus eliminates butterfly wing patterning." G3. jkac021.

Eleftherianos I, Zhang W, Heryanto C, Mohamed A, Contreras G, Tettamanti G, Wink M, Bassal T. (2021) "Diversity of insect antimicrobial peptides and proteins - A functional perspective: A review." Int J Biol Macromol 191:277-287.

Eleftherianos I, Heryanto C, Bassal T, Zhang W, Tettamanti G, Mohamed A. (2021) "Hemocyte-mediated immunity in insects: Cells, processes, and associated components in the fight against pathogens and parasites." Immunology 164(3):401-432.

Zhang W, Tettamanti G, Bassal T, Heryanto C, Eleftherianos I, Mohamed A. (2021) "Regulators and signalling in insect antimicrobial innate immunity: Functional molecules and cellular pathways." Cell Signal. 83:110003.

Eleftherianos I, Heryanto C. (2021) "Transcriptomic Insights into the Insect Immune Response to Nematode Infection." Genes (Basel). 12(2):202.

Tendolkar A, Pomerantz AF, Heryanto C, Shirk PD, Patel NH and Martin A. (2021) "Ultrabithorax Is a Micromanager of Hindwing Identity in Butterflies and Moths." Frontiers in Ecology and Evolution. doi.org/10.3389/fevo.2021.643661

Heryanto C, Eleftherianos I. (2020) "Nematode endosymbiont competition: Fortune favors the fittest." Mol Biochem Parasitol. 238:111298.

Heryanto C, Kenney E, Eleftherianos I. (2020) “A Workflow for Infection of Drosophila with Entomopathogenic Nematodes to Monitor Immune Gene Transcriptional Activity.” In: Sandrelli F., Tettamanti G. (eds) Springer Protocols Handbooks: Immunity in Insects, pp 2013-216. Humana, New York, NY.

Eleftherianos I, Heryanto C. (2020) "Molecular Regulators of Entomopathogenic Nematode-Bacterial Symbiosis." Results Probl Cell Differ. 69:453-468.

Chevée V, Sachar U, Yadav S, Heryanto C, Eleftherianos I. (2019) "The peptidoglycan recognition protein PGRP-LE regulates the Drosophila immune response against the pathogen Photorhabdus." Microb Pathog. 136:103664.

Harsh S, Heryanto C, Eleftherianos I. (2019) "Intestinal lipid droplets as novel mediators of host-pathogen interaction in Drosophila." Biol Open. 19;8(7):bio039040.

Patrnogic J, Heryanto C, Ozakman Y, Eleftherianos I. (2019) "Transcript analysis reveals the involvement of NF-κB transcription factors for the activation of TGF-β signaling in nematode-infected Drosophila." Imunogenetics. 71(7):501-510.

Cooper D, Wuebbolt C, Heryanto C, Eleftherianos I. (2019) "The prophenoloxidase system in Drosophila participates in the anti-nematode immune response." Mol Immunol. 109:88-98.

Patrnogic J, Heryanto C, Eleftherianos I. (2018) "Transcriptional up-regulation of the TGF-β intracellular signaling transducer Mad of Drosophila larvae in response to parasitic nematode infection." Innate Immun. 24(6):349-356.

Patrnogic J, Heryanto C, Eleftherianos I. (2018) "Wounding-induced upregulation of the Bone Morphogenic Protein signaling pathway in Drosophila promotes survival against parasitic nematode infection." Gene. 673:112-118.

Patrnogic J, Castillo JC, Shokal U, Yadav S, Kenney E, Heryanto C, Ozakman Y, Eleftherianos I. (2018) "Pre-exposure to non-pathogenic bacteria does not protect Drosophila against the entomopathogenic bacterium Photorhabdus." PLoS One. 13(10):e0205256.

BS of Applied Science, HAN University, The Netherlands, 2011.